Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells

Jia-Yu ZhaoDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

Jia-Qi WangDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China

Li WuDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, ChinaCorrespondence[email protected]

Feng ZhangDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

Zhi-Peng ChenDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

Wei-Dong LiDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

Hao CaiDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

Xiao LiuDepartment of Pharmacology, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing210023, China;National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing210023, China

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Abstract

The study was intended to investigate the protective effects of emodin against cell injury and inflammation in AR42J cells. We determined trypsin and lipase activity, intracellular ROS and MMP using specific assay kits. The related protein expression and TNF-α and IL-6 in the medium were assayed by Western blot and ELISA kits. Results showed that emodin could protect AR42J cells against cell injury caused by cerulein and lipopolysaccharide which were possibly associated with inhibition of mitochondrial damage, ROS production, and then significantly inhibited ROS-mediated pathway, and ameliorated pancreatic cells injury by depleting the levels of inflammatory cytokines.

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Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells

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Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells

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