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Three new compounds, ascotrichols A-B (1–2) and ascotrichrone A (3), along with two known isocoumarin derivatives (4–5), were isolated from the solid culture of a sea mud-derived fungus, Ascotricha sp. ZJ-M-5 on rice media. The structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR data, and the absolute configurations were determined by electronic circular dichroism and biogenetic correlation.
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