Anti-inflammatory diterpenes from the fruits of Vitex trifolia L. var. simplicifolia Cham

Abstract

Two new labdane-type diterpenes, named viterotulin C (1) and vitexilactone D (2), together with five known diterpenes (3–7), were isolated from the fruits of Vitex trifolia L. var. simplicifolia Cham. Their structures were elucidated by detailed analysis of spectroscopic data. All the compounds were evaluated for their inhibitory effects on nuclear factor-kappa B (NF-κB) pathway in HEK 293 cell line. These compounds presented inhibition on TNF-α-induced NF-κB activation, with inhibition rates ranging from 42.52 ± 10.69% to 68.86 ± 10.76% at the concentration of 50 μM.

Keywords:

• Verbenaceae
• Vitex trifolia L. var. simplicifolia
• labdane-type diterpene
• anti-inflamatory activity
• nuclear factor-kappa B

Additional information

Funding

This work was supported by the State Key Program of National Natural Science Foundation of China [grant number 81630106]; the National High Technology Research and Development Program of China (863 Program) [grant number 2013AA093001]; the Ministry of Education of People’s Republic of China “Program for Innovative Research Team in University” [grant number IRT-16R54]; the Tianjin City High School Science & Technology Fund Planning Project [grant number 20140147]; and the Guangxi Colleges and Universities Key Laboratory of Zhuang medicine prescriptions basis and application research [grant number Gui Jiao Ke Yan [2014] zyfy201501].