Isolation of chemical constituents with anti-inflammatory activity from Reineckia carnea herbs

Abstract

Three new saponins (1–3), a new natural product (4) and six other known compounds (5–10) were isolated from the whole Reineckia carnea plant. Their structures were established by comparison of their NMR spectra and MS data with literature data. In addition, all the isolated compounds were evaluated in vitro for anti-inflammatory activities against LPS-stimulated nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 1–4 exhibited anti-inflammatory activities with IC₅₀ values of 37.5 µM, 31.4 µM, 34.6 µM, and 56.1 µM, respectively. Furthermore, compounds 5–10 showed anti-inflammatory activities with IC₅₀ values ranging from 20.3 to 42.9 µM.

Graphical Abstract

Keywords:

• Reineckia carnea
• structure identification
• anti-inflammatory activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by the National Natural Science Foundations of China [grant No. 81860684]; Jiangxi Province key R&D Projects [grant No. 20165BCB19009]; The Health and Family Planning Commission of Jiangxi Province [grant Nos. 2017A291, 20183009].