331
Views
1
CrossRef citations to date
0
Altmetric
Articles

Identification of a flavonoid 7-O-glucosyltransferase from Andrographis paniculata

, &
Pages 279-286 | Received 10 May 2019, Accepted 09 Oct 2019, Published online: 18 Nov 2019
 

Abstract

Andrographis paniculata is an important traditional medicinal herb in which flavonoids are part of the primary specialized metabolites. A flavonoid glucosyltransferase with broad substrate spectrum (named ApUGT3) was successfully identified by screening homologous glycosyltransferase genes from A. paniculata. The enzyme displayed glycosylation activity toward multiple flavonoids in vitro, and the major products were identified as 7-O-glucosides. Phylogenetic analysis revealed that ApUGT3 is the first reported glycosyltransferase from the Acanthaceae family that belongs to cluster I, suggesting that ApUGT3 is a new flavonoid glycosyltransferase of this subcluster. This enzyme is potentially useful as powerful glycosylation catalysts to modify flavonoid-like compounds and improve their biological activities.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by National Natural Science Foundation of China (81891010, 81891013, 81673547), the Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (Grant No. 2060302), and the Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th 5-year Plan (CIT&TCD20170324), and National Program for Special Support of Eminent Professionals.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.