Rapid identification and structural characterization of polyoxypregnane glycosides in Dregea sinensis by HPLC-MSⁿ and HRMS

Abstract

By HPLC-MSⁿ and HRMS analyses, the structures of 52 polyoxypregnane glycosides were rapidly inferred from Dregea sinensis Hemsl on the basis of their sodium-cationized molecules [M + Na]⁺ and predominant diagnostic ions resulting from the saccharic chain on C3 and the neutral loss of substituent on C11 and C12. Compounds 1 and 7 significantly inhibited LPS-stimulated splenocyte proliferation in vitro.

Keywords:

• Dregea sinensis
• polyoxypregnane glycosides
• structural analysis
• MS fragmentation pattern
• immunomodulatory activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Supporting information

HR-MS and ESI-MS/MSⁿ spectra of compounds 1–52 can be found in supporting information.

Additional information

Funding

This project was financially supported by the National Natural Science Foundation of China (Grant No. 81503353).