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Abstract
The strain which degraded steviol glycosides to steviol (STE) was screened and isolated from soil samples. A phylogenetic tree was constructed and used to determine the taxonomic status of the strain. 16S rDNA sequence was ultimately used to identify the strain as Microbacterium barkeri XJ. The transformation product was detected and identified as STE by HPLC/LC-MS/IR analysis. The bioconversion rate of 1% (v/v) steviol glycosides (stevioside, rebaudioside A, rebaudioside C) into STE in basic medium were 100% within 24 h, 84 h and 144 h, respectively. The results indicated XJ was more effective than mixed flora in the bioconversion of steviol glycosides to STE.
Graphical Abstract
Disclosure statement
No potential conflict of interest was reported by the author(s).
Additional information
Funding
This work was financially supported by the National Key Research and Development Program of China (2019YFA0904900), the Program for Jiangsu Excellent Scientific and Technological Innovation Team (17CXTD00014), the High Level Talent Project of the 12th Batch of “Six Talents Summit” in Jiangsu Province (NY-011-2015) and the Special Projects of Introduction of Foreign Technical and Managerial Talents (BX2019014).