Constituents of Tinospora sinensis and their nitric oxide inhibitory activities

Abstract

One new phenylpropanoid glycoside, tinosinen A (1) and 13 known compounds, tinosinen (2), citrusin B (3), picraquassioside C (4), erythro-guaiacylglycerol-β-O-4′-coniferyl alcohol (5), erythro-guaiacylglycerol-8-O-4′-(sinapyl alcohol) ether (6), erythro-syringylglycerol-8-O-4′-(sinapyl alcohol) ether (7), seco-isolariciresinol 9-O-D-β-glucopyranoside (8), tinosposide A (9), pinoresinol-4′-O-β-D-glucopyranoside (10), syringaresinol-4′-O-β-D-glucopyranoside (11), pinoresinol (12), syringaresinol (13), and lirioresino-β-dimethyl ether (14) were isolated from the stems of Tinospora sinensis (Lour.) Merr. Their structures were established by detailed spectroscopic studies and comparisons with those reported in the literature. Compound 13 showed significant inhibitory NO production (IC₅₀ value of 38.53 ± 1.90 μM) in RAW264.7 macrophages, LPS-stimulated. Compounds 3–7, 11, 12, and 14 inhibited NO production with IC₅₀ values ranging from 38.53 to 99.07 μM.

Graphical Abstract

Keywords:

• Tinospora sinensis
• Menispermaceae
• phenylpropanoid glycoside
• tinosinen A
• nitric oxide inhibitory

Disclosure statement

No potential conflict of interest was reported by the author(s).

Figure 1. The chemical structure and the key HMBC and COSY correlations of compound 1.

Additional information

Funding

This research was financially supported by Vietnam Ministry of Education and Training under grant number B2021-GHA-02.