Regulatory effect of isovitexin on MAPK/NF-κB signal in mice with acute ulcerative colitis

Abstract

The aim of this study was to investigate the anti-inflammatory effects and mechanism of isovitexin on ulcerative colitis mice and RAW264.7 cells. The results showed that isovitexin had strong antioxidant and anti-inflammatory effects, and could restore intestinal barrier integrity (p < 0.01). In addition, isovitexin inhibited the expression of MyD88, TLR4 and NF-κB p65 proteins. At the same time, isovitexin can inhibit the activation of MAPK/NF-κB signaling pathway in RAW264.7 cells. In conclusion, isovitexin has a protective effect on UC mice, and its improvement mechanism of UC might be related to MAPK/NF-κB signaling pathway.

Graphical Abstract

Keywords:

• Isovitexin
• ulcerative colitis
• MAPK/NF-κB signaling pathway

Ethical approval

This research was conducted according to approved animal protocols and guidelines established by the Experimental Animals of Yunnan University of Traditional Chinese Medicine (Kunming, China) (Approval Number: SYXK (Dian) K2017-0005). We have done our best to minimize animal suffering and to reduce the number of animals used.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

All datasets generated for this study are included in the article.

Additional information

Funding

This research was financially supported by the National Natural Science Foundation of China [No. 81960777], the National Natural Science Foundation of China (Yunnan Ten-thousand Talents Program to Y.-P.L.; the Union Special Fund of Yunnan Provincial Science and Technology Department and Yunnan University of Chinese Medicine [No. 2019FF002(-005)].).