Trans-ε-viniferin as an inhibitor of TMEM16A preventing intestinal smooth muscle contraction

Abstract

TMEM16A regulator is an important tool to study the physiological functions and pathogenesis related to TMEM16A. In the present study, trans-ε-viniferin (TV) was identified as a TMEM16A inhibitor with inhibitory activity against TMEM16A mediated Cl⁻ currents, which was reversible, without affecting intracytoplasmic Ca²⁺ concentration and TMEM16A protein expression. TV inhibited intestinal peristalsis and prolonged gastrointestinal transport time. TV could inhibit autonomic and E_act-stimulated intestinal contractility, and was equally effective in ACh- and HA-induced high contractile states. The results indicate that TV significantly inhibits the intestinal smooth muscle contraction, which may be applied in the treatment of TMEM16A-related intestinal dynamic abnormalities.

Graphical Abstract

Keywords:

• Trans-ε-viniferin
• TMEM16A
• inhibitor
• smooth muscle contraction

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was financially supported by Scientific Research Project of Liaoning Province Education Department [grants No. LJKMZ20221432].