C-27 and C-3 Glucosylation of Diosgenin by Cell Suspension Cultures of Costus Speciosus

Abstract

3-O-[β-D-glucopyranosyl-(1″ → 2′)-β-D-glucopyranosyl], 27-O-β-D-glucopyranosyl-(25R)-spirost-5-ene-3β,27-diol was isolated from cell suspension cultures of Costus speciosus, following incubation with diosgenin, and its structure was elucidated using a combination of one- and two-dimensional ¹H and ¹³C NMR spectral data, and positive and negative ion ESMS spectral data.

Key Words:

• Costus speciosus
• Cell suspension cultures
• Biotransformation
• Glucosylation
• Diosgenin
• 3-O-[β-D-glucopyranosyl-(1″ → 2′)-β-D-glucopyranosyl]
• 27-O-β-D-glucopyranosyl-(25R)-spirost-5-ene-3β,27-diol