Carbazole alkaloids as new cell cycle inhibitor and apopTosis inducers from Clausena dunniana Levl

Carbazole alkaloids, 3-methylcarbazole (1), murrayafoline A (2), girinimbine (3), mahanimbine (4) and bicyclomahanimbine (5), were isolated for the first time from Clausena dunniana Levl. by bioassay-guided separation procedure and were identified by spectroscopic methods. Compounds 1 - 5 showed growth inhibitory activity (1, IC₅₀ 25 w g/ml) on human fibrosarcoma HT-1080 cells and cell cycle M-phase inhibitory (2, MIC 0.78 w g/ml) and apoptosis inducing (2, MIC 1.56 w g/ml; 3, MIC 25 w g/ml; 4, MIC 20 w g/ml; 5, MIC 30 w g/ml) activities on mouse tsFT210 cells, respectively, and 2 - 5 provided the first examples of carbazole alkaloids as new cell cycle inhibitors and apoptosis inducers.

Keywords:

• Rutaceae
• Clausena Dunniana
• Carbazole Alkaloid
• 3-Methylcarbazole
• Murrayafoline A
• Girinimbine
• Mahanimbine
• Bicyclomahanimbine
• Cell Cycle Inhibitor
• Apoptosis Inducer