Diosgenin-3-O-α-l-Rhamnopyranosyl-(1→4)-β-d-glucopyranoside obtained as a new anticancer agent from Dioscorea futschauensis induces apoptosis on human colon carcinoma HCT-15 cells via mitochondria-controlled apoptotic pathway

Abstract

Diosgenin-3-O-α-l-rhamnopyranosyl-(1→4)-β-d-glucopyranoside (DRG) is a well-known pentacyclic triterpene glycoside newly isolated from the rhizomes of Dioscorea futschauensis R. Kunth (Dioscoreaceae) by our group. In the present work, the inhibitory effect of DRG on the cell proliferation of human cancer cell lines was examined to reveal for the first time that DRG shows stronger anticancer activity than that of the positive control cisplatin. DRG inhibited the proliferation of human cancer cells, A431, A2780, A549, K562, and HCT-15, with IC⁵⁰ (μmol L^-1) values of {\rm 9.33 \pm 0.22,} {\rm 18.7 \pm 0.16,} {\rm 9.98 \pm 0.38,} {\rm 6.44 \pm 0.10,} and {\rm 5.86 \pm 0.14} respectively. It was then found, by morphological observation, “DNA ladder” detection and flow cytometric analysis, that DRG exerts its anticancer effect through inducing apoptosis on HCT-15 cells. Furthermore, it has been demonstrated that DRG triggers a mitochondria-controlled apoptotic pathway to induce apoptosis on HCT-15 cells, which involves the reduction of the mitochondrial potential (Δψ ^m), the release of cytochrome c from mitochondria into the cytosol, and the down-regulation of the ratio of Bcl-2/Bax expression level. The present results reasonably suggest that regulating the balance of Bcl-2/Bax expression level plays a key role in the DRG-induced apoptosis. Such findings provide novel knowledge to elucidate the biological properties of DRG, even though DRG was discovered early in the late 1960s. These results suggest that DRG may be a good candidate as a chemotherapeutic agent to treat human colon carcinoma.

Keywords:

• Diosgenin-3--α--rhamnopyranosyl-(1→4)-β--glucopyranoside
• Apoptosis
• Bax
• Bcl-2
• Cytochrome
• Mitochondrial transmembrane potential
• HCT-15 cells

Acknowledgements

The authors are grateful to Dr Takeshi Tsuruo, Professor of Tokyo University, for his kind gifts of cancer cell lines. This work was supported by the fund from the National Natural Science Foundation of China (C.-B. Cui, No. 39825126) and the fund from the Ministry of Science and Technology (C.-B. Cui, No. G199805113), China.