84
Views
9
CrossRef citations to date
0
Altmetric
Original Articles

Effect of leonurine on the activity of creatine kinase

, &
Pages 281-287 | Received 02 Jun 2003, Accepted 09 May 2003, Published online: 19 Aug 2006
 

Abstract

The effects of leonurine (1) on the activity of creatine kinase (CK) have been studied. The results show that leonurine inhibits enzyme activity in concentration- and time-dependent manners (at 0.75 and 1.51 mmol from 12 to 72 h). There are two mechanisms for the inhibition process. Compound 1 first acts as a non-competitive inhibitor and then as an irreversible inhibitor. Changes of CK were not found in 10% SDS-PAGE, but the amount of dimeric CK decreased in 10% non-SDS gel. The results suggest that 1 can inhibit CK activity by degrading its dimeric structure.

Acknowledgements

The authors thank Drs Zhou Hai-Meng, Yu Zhen-Hang and Ms Wang Yu for their support during this study. This research was supported in part by the Tsinghua University–Hong Kong Baptist University Joint Institute for Research of Chinese Medicine and the Tsinghua University 985 Project.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.