Two new triterpenoid saponins from Pulsatilla cernua (Thunb.) Bercht. et Opiz

Abstract

Two new triterpenoid saponins, named cernuaside C and D, have been isolated from Pulsatilla cernua (Thunb.) Bercht. et Opiz. The structures of the two new triterpenoid saponins were elucidated as 3-O-β-D-xylopyranosyl(1 → 2)-[α-L-rhamnopyranosyl(1 → 3)]-α-L-arabinopyranosyl oleanolic acid 28-α-L–rhamnopyranosyl(1 → 4)-β-D-glucopyranosyl(1 → 6)-β-D-glucopyranoside. (1) and 3-O-α-L–arabinopyranosyl (1 → 3)-α-L-rhamnopyranosyl(1 → 2)-α-L-arabinopyranosyl oleanolic acid 28–O–β-D-glucopyranoside (2) by 1D, 2D-NMR techniques, ESI-MS analysis as well as chemical methods.

Keywords:

• Ranunculaceae
• Pulsatilla cernua (Thunb.) Bercht. et Opiz
• Triterpenoid saponin
• Cernuaside C and D

Acknowledgements

The authors are grateful to Supported by Program for Changjiang Scholars and Innovative Research Team in University, IRT0413 and China International Science and Technology Cooperation programme (2006DFA31230).