![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
A new acetyl flavonol, named 3-O-acetyl-7-O-methyl kaemferol (1), together with the five known compounds rhamnocitrin (2), rhamnocitrin-3-O-β-d-glucoside (3), rhamnocitrin-4′-β-d-glucoside (4), rhamnazin (5), and p-hydroxyl benzoic acid (6) was isolated from Nervilia fordii (Hance) Schltr. The structures of the compounds were determined by spectroscopic analysis. All the compounds were evaluated against nitric oxide (NO) release, based on the production of NO in mice RAW264.7 stimulated by lipopolysaccharide (LPS). This new compound (1) showed potent inhibitory activity against the production of NO in RAW264.7 stimulated by LPS with the IC50 value of 16.79 μM.
Acknowledgements
The project was funded by the Key Laboratory of Medicinal Chemical Resources and Molecular Engineering from Guangxi Normal University, and the funding from Guangdong Natural Science Foundation, China. All the spectroscopic data were acquired from the Institute of Traditional Chinese Medicine and Natural Products, Jinan University. The authors are grateful to Mr Wei-Hua Jiao and Ms Yan Yu for the measure of all NMR spectra, to Mr Cheng-Yan Li for the measure of LR-ESI-MS, and to Mr Chun-Lin Fan for the measure of HR-ESI-MS. We also express our thanks to Dr Feng Zhao at College of Pharmacy, Yantai University for the bioassay.