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Original Articles

Isolation of two new C-glucofuranosyl isoflavones from Pueraria lobata (Wild.) Ohwi with HPLC–MS guiding analysis

Original Article

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Pages 293-299 | Received 08 Dec 2009, Accepted 02 Mar 2010, Published online: 21 Apr 2010
 

Abstract

A profiling analysis of the total isoflavone extract of the root of Pueraria lobata (Wild.) Ohwi was performed using HPLC coupled with ESI-ion trap mass spectrometry. A total of seven isoflavones were identified according to their retention times, UV, MS data, and comparing with the literature data. Among them, two proposed new compounds were isolated and their structures were determined to be 8-C-α-glucofuranosyl-7,4′-dihydroxyisoflavone and 8-C-β-glucofuranosyl-7,4′-dihydroxyisoflavone, named as neopuerarin A (7) and neopuerarin B (6), on the basis of chemical and spectral analyses.

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