PRELIMINARY ASSESSMENT OF POLYCYCLIC AROMATIC HYDROCARBON MULTI-ANALYTE TETROL ISOMERS FROM SERUM ALBUMIN ADDUCTS BY GAS CHROMATOGRAPHY/HIGH RESOLUTION MASS SPECTROMETRY

Abstract

We have developed a preliminary method for the qualitative assessment of polycyclic aromatic hydrocarbon (PAH) tetrol metabolites in humans using serum albumin adducts. The method was used to investigate the presence of tetrol metabolites of PAHs released following acid hydrolysis of serum albumin-diol epoxide adducts. Liquid-liquid extraction and solid phase extraction were used for isolation and purification of the tetrol metabolites. Purified tetrols were derivatized to convert them to their trimethylsilyl derivatives and analyzed using gas chromatography/high resolution mass spectrometry. A total of 26 tetrol isomers were investigated, resulting in observation of the following 8 tetrols in 3 smoking and 7 non-smoking donors: 5-methylchrysene-1,2,3,4-anti-tetrol, benz(a)anthracene-1,2,3,4-anti-tetrol, benz(a)anthracene-1,2,3,4-syn-tetrol, benz(a)anthracene-8,9,10,11-anti-tetrol, (±)-benzo(a)pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (BPTI-1), (±)-benzo(a)pyrene-r-7,t-8,c-9,t-10-tetrahydrotetrol (BPTII-1), (±)-benzo(a)pyrene-r-7,t-8,c-9,10-tetrahydrotetrol (BPTII-2), and (±)-benzo(a)pyrene-r-7,t-8,9,10-tetrahydrotetrol (BPTI-2). This preliminary work presents the first reported method for the assessment of PAH multi-analyte tetrol isomers extracted from serum albumin adducts in human donors.

Keywords:

• albumin
• 5-methylchrysene tetrol
• benz(a)anthracene tetrol
• PAH tetrols
• polycyclic aromatic hydrocarbons

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The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.