Polycyclic Aromatic Hydrocarbons, Heavy Metals, and Genotoxicity of the Suburban Soils from Guangzhou, China

Abstract

During the past few decades, urban and suburban developments have grown at unprecedented rates and extents with unknown consequences for ecosystem function. The problem of soil pollution as a result of the accelerating development of Guangzhou in China is becoming great concerns. In the present study, gas chromatograph coupled mass spectrometry (GC-MS), inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectrometry (ICP-AES) were employed to determine the 16 US Environmental Protection Agency (EPA) priority polycyclic aromatic hydrocarbons (PAHs) and the heavy metals (As, Cr, Cu, Pb, Cd, Hg, and Se) of soils collected from suburban areas of Guangzhou. The genotoxicity of these soils was screened with micronucleus (MN) assay in Vicia faba root cells. The concentrations of the pollutants in the soils were (dried weight): ΣPAHs (230.6–1263 ng·g⁻¹), As (2282.6–36064 μg·kg⁻¹), Cr (7109–64699 μg·kg⁻¹), Cu (7047–56388 μg·kg⁻¹), Pb (9675.9–93739 μg·kg⁻¹), Cd (68.5–847.3 μg·kg⁻¹), Hg (85.4–549.2 μg·kg⁻¹), and Se (219.2–968 μg·kg⁻¹), which fell in the moderately polluted range. However, six out of nine soil-exposed groups had a significant increases of MN frequencies observed in the V. faba root cells compared with the negative group (P < 0.05, P < 0.01), indicating that they had potential genotoxic risks. Bringing together the chemical analyses with the biological effects observed in this study, the genotoxic response could at a certain degree be explained by both the soil PAHs and heavy metals. Our results suggested that apart from chemical analysis, bioassays like the MN assay of V. faba root cells should also be included in a battery of tests to assess the eco-environmental risks of urban and/or urbanization in the developing areas on the soils.

Key Words:

• genotoxicity
• heavy metal (HM)
• micronucleus (MN)
• polycyclic aromatic hydrocarbon (PAH)
• soil

Acknowledgments

This research was funded by Natural Science Foundation of China (41025012), Nature Science Foundation of Hunan Province (05JJ40021), Foundation of Educational Department of Hunan Province (12A118) and University of South China (2012XQD44), China.