Green Synthesis and Anticancer Evaluation of Novel Chrysin Hydrazone Derivatives

Abstract

Chrysin, a natural flavonoid, has been investigated for its anticancer biological properties. Antifungal and anticancer properties of hydrazone derivatives have been proven. In this study, eight compounds of substituted Chrysin hydrazine derivatives have been designed, synthesized, and screened against cancerous cell lines, including MCF-7 and HepG-2. All products are reported for the first time. Most compounds exhibited significant cytotoxic activity against selected cell lines. The chemical structures of all compounds were confirmed by spectroscopic methods and elemental analyzes. In vitro evaluation of these synthesized compounds showed most of them have potential antiproliferative activity. Compounds 3e and 3f have shown significant anti-proliferative activity against MCF-7 and HepG-2 cell lines, respectively, compared to Chrysin (P < 0.05). The IC₅₀ values of compounds 3e and 3f were 101.44 and 166.19 µM after 48 h, respectively. Moreover, the compounds 3e and 3f induced significant apoptosis more than Chrysin (P < 0.05). The Results of this research might help the design and synthesis of novel drugs toward developing new antitumor agents for cancer treatment.

Keywords:

• Chrysin
• anticancer activity
• IC₅₀
• apoptosis
• flavonoid

Acknowledgments

The authors express appreciation to the Student Research Committee, Jiroft University of Medical Sciences for supporting this investigation.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.