Effect of Caloric Restriction on Metabolism of Polycyclic Aromatic Hydrocarbons by Male B6C3F₁ Mouse Liver Microsomes

Abstract

Effect of caloric restriction (CR) on metabolism of 7-fluorobenz-[a]anthracene (7-F-BA), 7-chlorobenz[a]anthracene (7-Cl-BA), 7-bromo-benz[a]anthracene (7-Br-BA), and 7-nitrobenz[a]anthracene (7-nitro-BA) by mouse liver microsomes was studied. The results indicate that CR can affect metabolism rate, modulate the regioselectivity and stereoselectivity of the cytochrome P-450 metabolizing enzymes, and lower the yield of the proximate mutagenic metabolites. These findings indicate that CR may in part modulate chemical carcinogenesis by altering the metabolic activation.

Key Words:

• Caloric restriction
• 7-fluorobenz[a]anthracene
• 7-chlorobenz[a]-anthracene
• 7-bromobenz[a]anthracene
• 7-nitrobenz[a]anthracene
• mouse liver microsomal metabolism.