Identification of Dibenzo[a,l]pyrene-DNA Adducts Formed in Cells in Culture and in Mouse Skin

Abstract

The DNA adducts formed from the potent carcinogen dibenzo[a,l]pyrene (DB[a,l]P) in cultures of Sencar mouse embryo cells and a mouse keratinocyte cell line and in Sencar mouse epidermis in vivo were analyzed by ³⁵S−phosphorothioate postlabeling, immobilized boronate chromatography and reverse-phase HPLC. The results demonstrate that the major DNA adducts result from the activation of DB[a,l]P to DB[a,l]P-11,12-diol-13,14-epoxide. Adducts from both the syn− and anti−isomers of this diol epoxide are formed in cells in culture, but in mouse epidermis only anti−isomer adducts are detected.

Key Words:

• DB[a,l]P
• DNA adducts
• cell culture
• mouse skin
• DB[a,l]PDE
• postlabeling