Advances in the Extraction, Purification and Detection of the Natural Product 1-Deoxynojirimycin

Abstract

1-Deoxynojirimycin (1-DNJ), a polyhydroxylated alkaloid, is a highly selective and potent glycosidase inhibitor that has garnered great interest as a tool to study cellular recognition and as a potential therapeutic agent. The development of analytical methods for the quantification polyhydroxylated alkaloids in natural products requires a multifaceted approach. Many publications over the past five decades have described analytical methods for this compound. However, recently more advanced techniques have come to prominence for sample extraction, purification, detection, and identification. This review provides an updated, extensive overview of the available methods for the extraction, purification, identification or detection of 1-DNJ. The review highlights different strategies for the design of 1-DNJ detection methods, which we analyzed in light of recent detection data. Finally, we conclude with perspectives on possible strategies for increasing the efficiency of identification and quantification of 1-DNJ in the future.

Keywords:

• 1-Deoxynojirimycin
• detection
• extraction
• glycosidase inhibitors
• identification
• purification

Compliance with ethical standards

Ethical approval This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Funding

The research was financially supported by the National Natural Science Foundation of China (NSFC-21776209 and NSFC-21621004), Natural Science Foundation of Tianjin (No. 19JCYBJC21100), Science and Technology Plan Project in Sichuan Province (2019YFN0114) and Financial Innovation Ability Promotion Project in Sichuan Province (2016TSCY-006).