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Abstract
The essential oil of Hedychium spicatum rhizome is used in perfumery and medicines. The rhizome is reported to be stomachic, carminative, a bronchodilator stimulant and a tonic. For studying seasonal variation of the composition of its volatiles, rhizomes of H. spicatum were collected from the Council of Scientific and Industrial Research–Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur, Himachal Pradesh, India. To study the spatial diversity in essential oil content and composition, the rhizomes were collected from five different locations of Himachal Pradesh. Essential oils obtained by hydro-distillation were evaluated for their chemical composition by gas chromatography (GC) and GC/mass spectrometry (GC/MS) analyses. Twenty-two compounds were identified from the rhizome oil with major components 1,8-cineole, β-eudesmol, β-pinene and 10-epi–γ-eudesmol. All the essential oil samples were evaluated for their antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. The essential oil extracted from the rhizomes collected from Biling had highest antioxidant activity; hence, it was also tested for cytotoxic activity against C-6, A549 and SiHa cell lines.
Acknowledgments
The authors appreciatively acknowledge the Director of the IHBT (Council of Scientific and Industrial Research) Palampur, for continuous support and for providing the necessary facilities during the course of the analyses. The authors are also grateful to Mrs. Vijaylata Pathania for providing GC and GC/MS data., and to CSIR, New Delhi, for funding CSIR-800 RSP-0021, BSC-106 and BSC-110 projects under which the crop was cultivated at different locations in HP and analyzed. IHBT Publication number: 3440.
