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Articles

Effect of melatonin, salicylic acid and gibberellic acid on leaf essential oil and other secondary metabolites of bitter orange young seedlings

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Pages 487-496 | Received 26 May 2014, Accepted 16 Jun 2015, Published online: 22 Jul 2015
 

Abstract

An investigation was carried out in order to estimate the biochemical changes occurring in bitter orange (Citrus aurantium L.) leaves in response to melatonin (MEL), gibberellic acid (GA) and salicylic acid (SA) treatments. We examined the effect of MEL, GA and SA on the essential oil (EO), the total phenolic (TPC) and total flavonoid content (TFC), and the antioxidant activity of bitter orange leaves from 1-year-old plants. 15 μMMEL, 1 mMSA and 1.44 mMGA increased the total leaf phenolic and flavonoid content and enhanced FRAP and DPPH activity of leaf methanolic extracts. Salicylic acid and GA promoted the leaf essential oil content and affected significantly the concentration of limonene, linalool and linalyl acetate.

Acknowledgements

This work was supported financially by the Aristotle University of Thessaloniki. We would also like to express our sincere thanks to Sofia Kuti, Vasiliki Tsakiridou and Eleni Lalidou for technical assistance.

Disclosure statement

No potential conflict of interest was reported by the authors.

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