Chemotypic characterization of diversity in essential oil composition of Ocimum species and varieties from India

ABSTRACT

Chemical composition of essential oils isolated from four species of Ocimum (Ocimum basilicum L., Ocimum × citriodorum Vis., Ocimum kilimandscharicum Gurke, Ocimum. viride Willd.) and six botanical varieties of Ocimum basilicum L. (O. basilicum var. difforme, O. basilicum var. purpurascens, O. basilicum var. basilicum, O. basilicum var. pilosum, O. basilicum var. glabratum and O. basilicum var. thyrsiflora) were investigated by GC/FID and GC/MS. Results revealed a total of sixty-four volatile compounds showing both qualitative and quantitative variations among these species/varieties in distribution of compounds, chemical profiles and chemotypes. On the basis of chemical composition and hierarchical clustering, Ocimum species/varieties were classified into seven different chemotypes. Ocimum × citriodorum showed two different chemotypes rich in geranial/neral and methyl chavicol. O. kilimandscharicum showed camphor-rich chemotype (43.21%), while O. viride was found to be eugenol-rich chemotype (77.86%). Among the O. basilicum varieties, linalool-rich chemotype was identified in thyrsiflora, difforme, purpurascens and glabratum, whereas methyl chavicol-rich chemotype was found in pilosum. The most commonly found species of O. basilicum var. basilicum showed four chemotypes: linalool, methyl chavicol, (E)-methyl cinnamate and mixed type having both linalool and methyl chavicol. These essential oils of Ocimum with varying chemical compositions and chemotypes can be utilized in perfumery, pharmaceutical, food preservation and aromatherapy industries.

KEYWORDS:

• Ocimum species/varieties
• O. basilicum L. var. thyrsiflora
• O. basilicum L. var. difforme
• O. basilicum L. var. purpurascens
• O. basilicum L. var. glabratum
• O. basilicum L. var. pilosum
• Ocimum × citriodorum
• Ocimum kilimandscharicum
• Ocimum viride
• essential oil composition
• chemotype
• GC/FID
• GC/MS

Acknowledgments

The authors are thankful to Late Dr. K.S. Negi for his contribution during the experimental work carried out at ICAR-NBPGR, Bhowali for the taxonomic identification of Ocimum species/varieties and the Director, ICAR-NBPGR, New Delhi for providing the necessary facilities. Special thanks to Dr. Rakesh Singh, Principal Scientist, DGR, ICAR-NBPGR, New Delhi for helping us in data analysis.

Disclosure statement

No potential conflict of interest was reported by the authors.