Abstract
The cDNA clones for five novel isoforms of prostaglandin (PG) F 2 f receptor (FP) were isolated from the bovine corpus luteal cDNA library. The sequence analysis revealed that each FP isoform, respectively, encodes 325 ( f ), 326 ( g ), 339 ( n ), 293 ( i ), and 298 ( l ) amino acid proteins, carrying the specific carboxyl tails (C-tails) caused by alternative mRNA splicing. Depending on the splicing sites, FP isoforms can be classified into two types. The FP- f, FP- g, and FP- n belong to the same group (Type I), which has common amino acid sequences with the exception of each specific 4th intracellular C-tail, while FP- i and FP- l are classified into the second group (Type II), which has a truncated structure lacking transmembrane domain VII and an intracellular C-tail. The RT-PCR analysis indicated that the mRNAs for these isoforms are expressed at the same time with that of FP in the bovine corpus luteum during the estrous cycle and pregnancy.