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Research Article

Cloning and Characterization of the Novel Isoforms for PGF 2α Receptor in the Bovine Corpus Luteum

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Pages 307-311 | Published online: 11 Jul 2009
 

Abstract

The cDNA clones for five novel isoforms of prostaglandin (PG) F 2 &#102 receptor (FP) were isolated from the bovine corpus luteal cDNA library. The sequence analysis revealed that each FP isoform, respectively, encodes 325 ( &#102 ), 326 ( &#103 ), 339 ( &#110 ), 293 ( &#105 ), and 298 ( &#108 ) amino acid proteins, carrying the specific carboxyl tails (C-tails) caused by alternative mRNA splicing. Depending on the splicing sites, FP isoforms can be classified into two types. The FP- &#102, FP- &#103, and FP- &#110 belong to the same group (Type I), which has common amino acid sequences with the exception of each specific 4th intracellular C-tail, while FP- &#105 and FP- &#108 are classified into the second group (Type II), which has a truncated structure lacking transmembrane domain VII and an intracellular C-tail. The RT-PCR analysis indicated that the mRNAs for these isoforms are expressed at the same time with that of FP in the bovine corpus luteum during the estrous cycle and pregnancy.

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