Molecular Cloning and Complete cDNA Sequence of UBH1 in Mouse Testis

Kwang-Hyun Baek College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea

Kyu-Hyung Park College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea

Yong-Soo Kim College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea

Myung-Sun Kim College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea

Hee-Kyung Choi College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, CHA General Hospital, Seoul, South Korea

Abstract

We have identified a full-length mouse UBH1 cDNA, encoding a putative deubiquitinating enzyme, from the testis by RT-PCR using primers prepared from sequences conserved amongst deubiquitinating enzymes. Sequence analysis predicts that the UBH1 cDNA encodes a 355 amino acid polypeptide with the molecular weight of approximately 39 kDa containing the highly conserved Cys, Asp, and His domains characteristic of the ubiquitin-specific processing proteases. Biochemical assay revealed that the mouse UBH1 has deubiquitinating enzyme activity and sequence analysis showed 98.3% amino acid identity with human UBH1.

Keywords

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Molecular Cloning and Complete cDNA Sequence of UBH1 in Mouse Testis

Information for

Open access

Opportunities

Help and information

Molecular Cloning and Complete cDNA Sequence of UBH1 in Mouse Testis

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature