cDNA Cloning and Expression of ATP Synthase Subunit b from the Fire-bellied Frog Bombina orientalis (Anura: Discoglossidae)

Min-Sun Kim Department of Life Science College of Natural Sciences, Hanyang University 133-791 Seoul South Korea

Il-Chan Kim Department of Biochemistry College of Medicine, Hanyang University 133-791 Seoul South Korea

Sang-Soo Kim Department of Life Science College of Natural Sciences, Hanyang University 133-791 Seoul South Korea

Sook-Kyoung Kim Department of Environmental Science, Graduate School Hanyang University 133-791 Seoul South Korea

Young-Mi Lee Department of Environmental Science, Graduate School Hanyang University 133-791 Seoul South Korea

Han Seung Kang Department of Life Science College of Natural Sciences, Hanyang University 133-791 Seoul South Korea

Hee-Seok Kweon Department of Environmental Science, Graduate School Hanyang University 133-791 Seoul South Korea

Ui-Wook Hwang Department of Biology Teachers College, Kyungpook National University 702-701 Taegu South Korea

Moon Kyoo Kim Department of Life Science College of Natural Sciences, Hanyang University 133-791 Seoul South Korea

Jae-Seong Lee Department of Environmental Science, Graduate School Hanyang University 133-791 Seoul South Korea

Yong-Dal Yoon Department of Life Science College of Natural Sciences, Hanyang University 133-791 Seoul South Korea

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Abstract

We cloned the Bombina orientalis adenosine triphosphate (ATP) synthase subunit b gene from a B. orientalis oviduct cDNA library. The transcript was 997 bp long and encoded 250 amino acid residues. It showed high similarity to amphibian (84-85%), mammalian (56-62%) and invertebrate (46-50%) sequences. In phylogenetic analyses, it clustered with other amphibian sequences. This gene was highly expressed in brain, intestine and oviduct but not in muscle and liver. In this paper, we report the basic characteristics of B. orientalis ATP synthase subunit b gene.

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cDNA Cloning and Expression of ATP Synthase Subunit b from the Fire-bellied Frog Bombina orientalis (Anura: Discoglossidae)

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cDNA Cloning and Expression of ATP Synthase Subunit b from the Fire-bellied Frog Bombina orientalis (Anura: Discoglossidae)

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