Abstract
The full-length cDNA (3612 bp) of OsNHA1 was cloned by RT-PCR approach from rice (Oryza sativa L.), which encodes a putative plasma membrane Na+/H+ antiporter. Its deduced protein, OsNHA1, has 11 transmembrane domains and a significant similarity to a plasma membrane Na+/H+ antiporter AtNHA1 from Arabidopsis thaliana. Phylogenetic analysis showed that the OsNHA1 clusters with the plasma membrane Na+/H+ antiporters from various organisms. The semi-quantitative RT-PCR assay revealed that the expression of OsNHA1 was up-regulated in both shoots and roots of rice seedlings under salt stress, whereas it was not induced in the rice seedlings treated by drought stress.
Acknowledgements
This work was supported by grants from Ph. D program foundation from Ministry of Education (20050307013) and the National Transgenic Plant Research and Commercialization Special Project of China (JY03A1101)
Notes
*Sequence data from this article have been deposited in GenBank/EMBL/DDBJ under Accession Number: AY328087