Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein

Abstract

We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.

• CDZFP
• subcellular localization
• northern blot
• DNA binding
• AY134856

Acknowledgements

We are grateful to Drs Songmin Jiang and Daojun Jiang for their revising this paper and helpful discussions. We also thank Drs Guangqing Cao for the gene sequencing. This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).