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Abstract
The nucleotide sequence of the internal transcribed region (ITS) of ribosomal RNA genes from Atlantic cod (Gadus morhua L.) was determined. The complete ITS region spanned approximately 1113 base pairs. The ITS1 region comprised 532 base pairs, the 5.8S region 159, and the ITS2 region contained 422 base pairs. Sequence data were obtained from a total of 12 samples, one pool from six cod and 11 individuals. The sequencing was carried out in two separate experimental periods employing slightly different methodology. The samples were from two different cod stocks, Norwegian costal cod and North East Arctic cod. The sequence analysis showed that in the 12 samples, the ITS region, including the 5.8S RNA, was identical. The ITS region is thus totally conserved in these two cod stocks. The extreme conservation of the ITS regions in the cod rDNA could reflect the small genome size of cod and/or indicate a specific critical role in the processing of the ribosomal units in cold-adapted species.
| Abbreviations | ||
| ITS | = | Internal transcribed spacer |
| rRNA | = | ribosomal RNA |
| rDNA | = | ribosomal DNA |
| bp | = | base pairs |
| Abbreviations | ||
| ITS | = | Internal transcribed spacer |
| rRNA | = | ribosomal RNA |
| rDNA | = | ribosomal DNA |
| bp | = | base pairs |
Acknowledgements
We would like to thank Gunnar Nævdal at the University of Bergen for valuable comments on previous drafts. The Norwegian animal research authority has approved the fish experiment described here. This report is a contribution from the ELOISE project: Effects of induced temperature change on marine coastal fishes (CLICOFI), funded by the European Union programme “Climate and environment”, contract No. ENV4-CT97-0596.