116
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

The Degradation Effects of a Pseudomonas Hydrolase OPHC2 to Organophosphorus Insecticides

, , &
Pages 804-810 | Published online: 20 Jun 2008
 

Abstract

The degradation effects of OPHC2 to 9 organophosphates and other pesticides were studied. The degrading reaction was carried out for 10 minutes under 37 °C in 50 mmol pH 8.0 Tris-HCl buffer. Organophosphates and sulfonylurea herbicides were detected by HPLC-UV and/or HPLC/MS. It's shown that degradation rates of 70%–100% were achieved for most of the organophosphates after 24 h (chlorpyrifos 100%, phoxim 100%, fenitrothion 99.87%, isocarbophos 100%, dimethoate 78.55%, methamidophos 71.55%, methidathion 87.93%). Pyrethroids were detected by GC-ECD after extracted by hexane. The degrading amounts of 5 pyrethroids were all above 50% after 3 hours (bifenthrin 78.7%, lambda-cyhalothrin 84.6%, permethrin 69.1%, beta-cypermethrin 51.5%, fenvalerate 72.2%). What is interesting, the degrading amount of malathion in its single standard solution was 94.31% while it did not degrade at all in the mixture standard even under superfluous enzyme solution. OPHC2 showed no effect to the sulfonylurea herbicides.

Acknowledgments

This research was supported by China National Basic Research and Development Program-2002CB410805.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.