Synthesis, Characterization of Some Transition-Metal Complexes of a New Heptadentate N₅S₂ Schiff-Base Ligand and the Effects of These Metal Complexes on U2OS Cells Cytotoxicity and DNA Cleavage Activity

Abstract

Metal complexes of a heptadentate N₅S₂ donor Schiff-base ligand were synthesized by the template reaction between 2,6-bis(2-aminothiophenoxymethyl)pyridine and 2,2 ′-bipyridine-6,6 ′-dicarboxaldehyde in the presence of Zn(II), Cd(II), Hg(II) and Pb(II) perchlorate salts. The complexes were characterized by IR, ¹ H NMR, MS-FAB⁺, and elemental analyses. The mitochondrial activity of U2OS cells after exposure to these complexes was determined by colorimetric assay, which detects the conversion of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) to formazon. DNA-binding activity of all complexes was also investigated using plasmid DNA pUC18 purified from E. coli.

Keywords:

• Mitocondrial activity
• MTT
• Schiff base
• template synthesis

Acknowledgments

Authors are grateful to Trakya University and Anadolu University, Commission of Scientific Research Projects.