Evaluation of the Biochemical Effects of Silyl-Phosphaalkenes on Oxidative and Nitrosative Stress Pathways Involving Metallocenters

GRAPHICAL ABSTRACT

Abstract

A series of tests is proposed for the evaluation of the effect of experimental drugs on oxidative and nitrosative stress pathways involving metallocenters, with silyl-substituted phospha-alkenes as test cases. Physiologically and pathologically relevant parameters of hemoglobin, myoglobin, red blood cells, and cytochrome c are explored, such as autooxidation oxygen affinity, lipid peroxidation, nitrite-induced oxidation, and enzyme-type turnover. Prooxidant and nitrosative-stress-related reactivities appear to some extent enhanced by the compounds examined here.

Keywords:

• Hemoglobin
• cytochrome c
• cisplatin
• silyl-phosphaalkenes
• oxidative stress

ACKNOWLEDGMENTS

Profs. Gabriela Nemes, Catalin Popescu, Graziella Turdeanu, and Lidia Varvari are thanked for helpful discussions. Dr. Bianca Voinescu is thanked for help in human blood sampling.

FUNDING

Financial support from the Romanian Ministry for Education and Research (PCCE 140/2008) and from a Ph.D. scholarship from Contract POSDRU/88/1.5/S/60185, “Innovative doctoral studies in a knowledge based society” to Cristina Bischin and Agota Bartok is gratefully acknowledged.