Induced apoptosis by mild hyperthermia occurs via telomerase inhibition on the three human myeloid leukemia cell lines: TF-1, K562, and HL-60

Abstract

The purpose of this research was to understand the effect of hyperthermia on the telomerase activity in human leukemic cell lines (HL-60, K562, and TF-1). The cells were treated by hyperthermia at the range of 41–44°C for 120 min and incubated for 96 h. Then telomerase activity, cell proliferation, and apoptosis were assessed. The results indicated that hyperthermia significantly induced apoptosis on the cells. The cells exhibited pre-apoptotic pattern at 41 and 42°C at 60–120 min and apoptotic pattern at 43 and 44°C over 30 min after hyperthermia. Telomerase activity (that was assayed immediately after hyperthermia) was stable at 41–42°C for 60 min but decreased to 35–40% at 120 min. However, at severe hyperthermia (43–44°C) telomerase activity was decreased in a time- and dose-dependent manner. Following hyperthermia (41–44°C up to 120 min), the cells were incubated for 96 h. In these conditions, the telomerase activity was decreased by about 60-80% in comparison with that untreated control cells.

Keywords:

• Mild hyperthermia
• telomerase activity
• human leukemic cells
• apoptosis
• differentiation