Transport of killifish Hypsolebias flagellatus: effects of salt use and previous feeding in association with transport time

ABSTRACT

The aim of the study was to evaluate survival and water quality during the transport of Hypsolebias flagellatus using different concentrations of salt in water and different transport times in association with different previous diets. In experiment I, 40 males (1.38 ± 0.83 g) were used and the tested salinities were: 0 (control), 2, 4 and 6 g of salt/L. Dissolved oxygen was the highest in the concentration of 4 g of salt/L and lower in the control treatment (P <.05). In experiment II, 30 males fed (0.68 ± 0.27 g) with commercial diet and 30 males (1.48 ± 1.00 g) fed with artemia were transported during 12, 24 and 48 hours. The survival after transport was lower in 48 hours (P <.05). The water pH was higher in animals fed with diet (P <.05). The water temperature and water total ammonia increased with increasing transport time (P <.05). Dissolved oxygen decreases regardless of diet (P <.05).

KEYWORDS:

• Cyprinodontiformes
• diet
• ornamental fish
• Rivulidae
• salinity

Acknowledgments

The present research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG-Brazil). LUZ, R.K. received a research grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq No. 308547/2018-7).

Compliance with ethical standards

Two transport experiments were carried out in accordance with the ethical standards of the Ethics Committee on Animal Use of UFMG (Comissão de Ética no Uso de Animais of UFMG protocols CEUA 173/2018 and 327/2018).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

Additional information

Funding

This work was supported by a research grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq No. 308547/2018-7); CNPq; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado de Minas Gerais.