Abstract
In this study, embryos of Sepiella japonica from eye primordium formation to the larval growing stage were collected and used for RNA-Seq analysis. A total of 183,542,186 clean reads were assembled de novo into 58,054 unigenes consisting of 54,118,228 bp, with the average length at 932 bp and the N50 at 1667 bp. 21,469 (36.98%) unigenes were annotated at least in one of four databases including non-redundant protein (NR), Swiss-Prot, clusters of orthologous groups of proteins (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 4460 (7.68%) unigenes were annotated in all databases. Analysis of differentially expressed genes (DEGs) was carried out on embryos at Eye primordium formation stage (SJ1), organ differentiation stage (SJ2), and hatching stage (SJ3). Overall, the current study provided the de novo assembly of S. japonica transcriptome and identified the DEGs and pathways during embryonic development, which will provide a fundamental genetic resource for further functional research.
Ethical approval
All animals received humane care as outlined in the Institutional Guidelines of the Care and Use of Laboratory Animals at Lingnan Normal University (Zhanjiang, China).
Author contributions
Guarantor of integrity of the entire study: Liyun Wang and Shaogang Li; study concepts: Liyun Wang and Shaogang Li; study design: Liyun Wang and Shaogang Li; definition of intellectual content: Liyun Wang and Daohai Chen; literature research: Liyun Wang, Shaogang Li, Lele Xu and Yongqin Li; clinical studies: Liyun Wang, Shaogang Li, Lele Xu and Yongqin Li; experimental studies: Liyun Wang, Shaogang Li, Lele Xu, Yongqin Li and Huaxu Chen; data acquisition: Liyun Wang and Shaogang Li; data analysis: Liyun Wang and Shaogang Li; statistical analysis: Liyun Wang and Shaogang Li; manuscript preparation: Liyun Wang and Shaogang Li; manuscript editing: Liyun Wang and Shaogang Li; manuscript review: Liyun Wang and Shaogang Li
Acknowledgments
The authors thank The Central Government Supports Local University-Marine Technology Innovation Team for experiment suggestions.
Disclosure statement
No potential conflict of interest was reported by the author(s).