A nonsynonymous SNP within the AMH gene is associated with litter size in Dazu black goats

Abstract

AMH, KISS1R and GDF9 genes play a vital role in human and animal reproduction and might be used as the genetic markers for the reproduction traits selection. The aim of this study was to screen the single nucleotide polymorphisms (SNPs) within the AMH, KISS1R and GDF9 genes and to determine the correlations between these SNPs and the litter size in goats. Nine single SNPs within these genes were used for genotyping of the 190 Dazu black goat populations by SNaPshot technique. The polymorphisms of nine SNPs within these genes were detected in Dazu black goats. The significant correlation was observed between one SNP (g.89172108A > C) within the AMH gene and the litter size of second born in Dazu black goats (p < 0.05). The SNP was located in exon 4 (XM_018050765.1) of the AMH gene and was one nonsynonymous substitution, which resulted in a change of an amino acid from Glutamine to Proline (Gln38Pro). These results suggested that the nonsynonymous SNP g.89172108A > C of AMH gene could be used as a potential genetic marker for Marker-assisted selection (MAS) in goats breeding programs.

Keywords:

• Single nucleotide polymorphisms
• AMH gene
• litter size
• Dazu black goats

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This project was financially supported the Fundamental Research Funds for the Central Universities [XDJK2019B049], the National Natural Science Foundation of China [No. 31301944], the Chongqing Overseas Student Entrepreneurship Innovation Support Plan [No. cx2019132], and the Chongqing Science and Technology Innovation Special Project [No. cstc2019jscx-gksbX0135].