Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide

Abstract

Mastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by transcriptome sequencing. The results showed that compared to the control group, the LPS treatment group had 121 up-regulated genes and 18 down-regulated genes. GO and KEGG enrichment analysis revealed that these differential genes were mainly enriched in the IL-17 signaling pathway, Legionellosis, Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, and other signaling pathways. Furthermore, the expression of GRO1 and CXCL3 mRNAs increased significantly after LPS treatment. These findings provide new insights for the treatment of mastitis in cows in the future.

Keywords:

• Cow mastitis
• LPS
• transcriptional sequencing technology
• MAC-T

Acknowledgments

This work was supported by Key Research and Development Project of Henan Province “Research on Key Technology of TCM substitution and its Application in Healthy Livestock Breeding” (221111111300) and Henan Province Major Science and technology project (231100310200).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Additional information

Funding

This work was supported by the Project of Tackling Key Problems in Science and Technology of Henan Province [212102110352, 212102110095], the Open Project Program of Beijing Key Laboratory of Traditional Chinese Veterinary Medicine at Beijing University of Agriculture [TCVM-201702], the Programmes of the National Beef Cattle and Yak Industrial Technology System [CARS-37].