Complete nucleotide sequence of the bovine β‐lactoglobulin gene

Abstract

In this paper we report the complete bovine β‐lactoglobulin gene sequence, consisting of seven exons within a total of 4724 base pairs. The inferred amino acid sequence agrees completely with that determined directly for the ? variant. The bovine β‐lactoglobulin gene sequence shows 89% homology to the corresponding ovine sequence, with complete conservation of gene organization and splice sites, though a significant proportion of CG dinucleotides have been lost since the species diverged. Comparison with the previously reported cDNA sequence reveals a number of nucleotide substitutions in addition to those which distinguish the A and ? variants. Three of these are silent mutations, however it appears that the occurrence in the cDNA sequence of a Val residue at position 105 may represent a new variant provisionally designated β‐lactoglobulin K, derived from β‐lactoglobulin A.

Notes

Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933

European Molecular Biology Laboratory, Heidelberg, Fed. Rep. Germany