Effect of Phytohormones on Micropropagation of Self-Heal (Prunella vulgaris L.)

Abstract

Self-heal (Prunella vulgaris L.), a medicinal plant with strong antiviral activities, was regenerated from seedling shoot-tips cultured on medium containing selected concentrations and combinations of various plant growth regulators. The highest frequency of multiple shoot regeneration was obtained with 3.0 mg L⁻¹ BA with 0.1 mg L⁻¹ IAA. Regeneration tests with leaf lamina, petiole, stem internodes, and root explants cultured on medium containing combinations of plant growth regulators at various concentrations were unsuccessful. Regenerated shoots obtained from shoot-tips readily rooted on media containing IAA, IBA, NAA, or 2,4-D, with medium containing 3.0 mg L⁻¹ IAA or 3 mg L⁻¹ IBA being the most effective. Rooted explants transferred to vermiculate in Magenta boxes and acclimated for 2 weeks could be planted into foam cups containing potting soil and maintained in an environmentally controlled plant growth room.

KEYWORDS:

• growth regulators
• in vitro culture
• organogenesis
• shoot proliferation
• shoot tip explants

Acknowledgments

The authors are grateful to The Scientific and Technological Research Council of Turkey (TUBITAK) for financial support [TBAG-2278(103T024) and TBAG-HD/27(105T040)]. Special thanks are expressed to Dr. Hakan Turker for his technical support.

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