In vitro Study of Tinospora cordifolia (Wild.) Miers (Menispermaceae)– a Multipurpose Plant, by Using Different Plant Bark Extracts for Secondary Metabolite Production

Abstract

Vegetative parts (stem, leaf, and nodal explants) of Tinospora cordifolia were excised from an in vivo grown mature plant and thereafter cultured on MS medium supplemented with 2, 4-D and bark extracts of Azadirachta indica and Acacia nilotica prepared in hexane, ethyl acetate, chloroform and methanol. The best callus growth was observed on MS medium supplemented with 2, 4-D (1 mg.L⁻¹) and A. indica bark methanolic extract (1 mg.L⁻¹). The methanol extracts of the dried callus showed the presence of alkaloids and sterols. HPLC, HPTLC, and GC-MS analysis of callus [MS+2, 4-D (1 mg.L⁻¹)] and callus [MS+2, 4-D (1 mg.L⁻¹) + A. indica methanolic extract (1 mg.L⁻¹)] were carried out for comparative study. The new-found peaks revealed the presence of methyl hexadecanoic acid with molecular weight of 270.

KEYWORDS:

• Barks extracts
• callus
• Secondary metabolites
• 2
• 4 –D