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ABSTRACT
Organogenesis of Carum copticum from hypocotyl explants of in vitro seedlings was examined. The highest callus induction was observed from hypocotyl explants on both Murashige and Skoog (MS) and Nitsch and Nitsch (NN) media. A maximum of 92% of hypocotyl explants produced callus on MS medium supplemented with 1.0 mgL–1 N6-benzyladenine (BA)and 0.5 mgL–1 naphthaleneacetic acid (NAA), whereas, 84% of hypocotyl explants produced callus on NN medium with 1.0 mgL–1 BA and 0.5 mgL–1 NAA. Hypocotyl explants-derived light green and friable callus responded to shoot organogenesis on both MS and NN media with plant growth regulators. A maximum of shoot regeneration frequency (76%) was observed on MS with 1.5 mgL–1 BA, whereas, 55% shoot regeneration frequency was observed on NN medium with 1.5 mgL–1 BA. A maximum number of 8.4 shoots per callus explant having a shoot length of 4.05 cm was observed on MS medium with 1.5 mgL–1 BA, whereas, 5.5 shoots per callus explant with a shoot length of 3.5 cm was observed on NN medium with 1.5 mgL–1 BA. Regenerated shoots were cultured on full strength MS and NN medium for root induction. The highest root regeneration frequency of 96% was obtained on MS medium with 0.25 mgL–1 NAA inducing 7.6 roots per shoot with root length of 4.25 cm, whereas, 83% root regeneration frequency was observed on NN medium with 0.25 mgL–1 NAA inducing 6.4 roots per shoot with a root length of 3.81 cm. In vitro derived plantlets were hardened and acclimatized successfully.
Acknowledgement
The authors express grateful thanks to Director, National Council of Education Research and Training, New Delhi and Principal, Regional Institute of Education, Bhopal for providing research facilities.