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ABSTRACT
In vitro multiplication and conservation protocol for Curcuma longa and Zingiber officinale using 25–100 mg L−1 carbendazim in MS basal and MS hormone medium was developed. Maximum enhancement in shoot proliferation was recorded on MS hormone supplemented with carbendazim at 25 mg L−1 giving in vitro conservation period of 6–12 months. Tissue culture derived one-year old field-grown turmeric plants showed normal vegetative growth and rhizomes formation. After 3 years of in vitro conservation, no differences were observed in plants conserved on BB25 medium and control for quantitative and qualitative traits and at genetic level as evidenced by SRAP profiles of turmeric and RAPD profiles of ginger.