Phenolic Contents, Antioxidant, and Antibacterial Activities of the Algerian Genista saharae Solvent Extracts

ABSTRACT

The phenolic compounds in solvent extracts of Genista saharae were analyzed by Reversed-phase high-pressure liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA) technique and evaluated for their antioxidant and antibacterial activities. The antioxidant activities were evaluated using hydrogen atoms transfer methods (DPPH, total antioxidant capacity, and reducing power assays) and single electron transfer (ABTS and cupric reducing antioxidant capacity assays). The antibacterial activity of extracts was tested against different strains using the minimal inhibitory concentration (MIC). Four phenolic acids (gallic acid, caffeic acid, p-coumaric acid, and sinapic acid), one flavonoid (quercetin), and one flavanone (naringenin) were identified with RP-HPLC. The chloroform extract exhibited antioxidant potential (IC₅₀ = IC₅₀ = 4.03 ± 0.0200 and 35.63 ± 1.42 µg L⁻¹) and antibacterial activity against the bacterial stains with MICs of 20 µg mL⁻¹. These results suggested a high contribution of quercetin and naringenin in the antimicrobial and antioxidant activities assessed.

KEYWORDS:

• RP-HPLC-PDA
• flavonoids

Disclosure of interest

The authors declare that they have no conflicts of interest concerning this article.

Acknowledgments

The authors are grateful to M^m Atik-Bekkara F, laboratory of Natural Product and Tlemcen-Algeria, director for his assistance.