ABSTRACT
The objective of this study was to evaluate the effect of combination of pheophorbide (Thyphonium flagelliforme) and andrographolide (Andrographis paniculata) on breast cancer cell culture of MCF-7 in a laboratory study using a posttest only control group design. human dermis fibroblast (HDF) cells were used as a control. Cell culture was obtained from YARSI University biorepository. Control and experimental treatment was given to six groups for each cell culture, namely control without treatment, P8 (pheophorbide 8 μM), A48 (andrographolide 48 μM), P2A12 (combination pheophorbide 2 μM and andrographolide 12 μM), P4A24 (combination pheophorbide 4 μM and andrographolide 24 μM), and P8A48 (pheophorbide combination 8 μM and andrographolide 48 μM). The treatment was given for 24 h, with three repetitions for each group. Results showed a decrease in cell viability in all treatment groups compared to the control group both in MCF-7 cells and HDF cells. Increasing the dose did not cause an increase in toxicity to MCF-7 cells. The use of a combination of pheophorbide and andrographolide showed anti-cancer effectiveness in MCF-7 breast cancer cell lines at 4 μM pheophorbide doses and 24 μM andrographolide
Acknowledgment
We would like to express our gratitude to YARSI University through the Internal Research Fund for conducting this research.