https://orcid.org/0000-0002-4167-7793
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ABSTRACT
The antihyperlipidemic and antioxidant effects of Mentha piperita leaves extracts in Poloxamer 407-induced hyperlipidemic rats were investigated. Antihyperlipidemic activities of hexane, ethyl acetate, and methanol extracts (100, 200, or 300 mg kg−1 b.w.) were evaluated by lipid profile analysis. Twelve (12) fractions (F1 to F12) were collected from the most antihyperlipidemic extract (ethyl acetate at 100 mg kg−1) and, assayed in vivo for antihyperlipidemic activity. The effects of the most antihyperlipidemic fraction (F2) on redox sensitive biomarkers (SOD, CAT, GSH, MDA) and lipoprotein lipase (LPL) activity at cellular and molecular levels were evaluated. Fraction-2 (F2) was further subjected to GC-MS and LC-MS analysis for identification of nonpolar and polar components. The F2 treatment decreased the level of MDA with concomitant enhancement of GSH, LPL activity in the liver and serum and up regulated the expression of SOD, CAT, and LPL genes in liver and blood relative to the untreated rats. GC-MS and LC-MS revealed oleic acid, vaccenic acid, 3, 4-dicaffeoylquinic acid, coumarin, and triacontane as the probable bioactive components with reported antihyperlipidemic and antioxidant effects.
KEYWORDS:
List of Abbreviations
| P407 | = | Poloxamer 407 |
| SOD | = | Superoxide dismutase |
| CAT | = | Catalase |
| GSH | = | Reduced glutathione |
| MDA | = | Malondialdehyde |
| LPL | = | lipoprotein lipase |
| GCMS | = | Gas Chromatography Mass Spectrometry |
| LCMS | = | Liquid Chromatography Mass Spectrometry |
| HPUR | = | hyperlipidemic untreated rats |
| CVDs | = | Cardiovascular diseases |
| BMI | = | body mass index |
| TC | = | total cholesterol |
| TG | = | triacylglycerol |
| LDL | = | low density lipoprotein |
| HDL | = | high-density lipoproteins |
| NC | = | Normal control |
| STZ | = | streptozotocin |
| RF | = | Retention factor |
| ATV | = | Atorvastatin |
| HRP | = | Horseradish peroxidase |
| TMB | = | Tetramethyl Benzidine |
| DF | = | Dilution factor |
| VLDL | = | Very Low Density Lipoprotein |
Acknowledgments
The authors appreciate the laboratory assistance of Mr. Aliyu and Mr. Ruben of the Department of Biochemistry Ahmadu Bello University, Zaria. We also thank Malam Aliyu and Malam Salihu of the Department of Pharmacology, Ahmadu Bello University, Zaria.
Authors’ contributions
Conception: Aliyu Muhammad, Hafsat Abdullahi Mohammed, Abdullahi Balarabe Sallau, Humphrey Chukwu Nzelibe and Mathew Kola Anigo
Design: Aliyu Muhammad, Hafsat Abdullahi Mohammed and Abdullahi Balarabe Sallau
Execution: Hafsat Abdullahi Mohammed, Ochuko Lucky Erukainure and Bashir Musa
Interpretation: Hafsat Abdullahi Mohammed, Ochuko Lucky Erukainure and Bashir Musa
Writing and Proof reading: Aliyu Muhammad, Hafsat Abdullahi Mohammed, Abdullahi Balarabe Sallau, Humphrey Chukwu Nzelibe and Mathew Kola Anigo
Disclosure statement
The authors declare no conflict of interest in this article.
Correction Statement
This article has been republished with minor changes. These changes do not impact the academic content of the article.