The Phytochemicals of Linum usitatissimum Seed Essential Oil Extracted by Hydrodistillation Induced Apoptosis and Suppressed Cell Migration in A549 Lung Cancer Cells

ABSTRACT

Flaxseed essential oil extracted by hydrodistillation was analyzed for its anticancer effects on A549 lung cancer cells. Phytochemicals were analyzed using Fourier-transform infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxicity effect was analyzed using Trypan blue, MTT, Clonogenic, DNA fragmentation, and wound scratch assays. Seventeen compounds with anticancer potency were identified. The cytotoxicity increased with increasing concentrations and inhibited migration of the cancer cells. Smeared DNA in gel and increased OD values compared to intact control DNA were also possible because of the phytochemicals in the essential oils.

KEYWORDS:

• A549 cell line
• growth inhibition
• anti-migratory effect

Acknowledgments

The technical support rendered by our colleagues Dr. Vani, an Assistant Professor, and Mr. Poomalai, a Lab Assistant, is gratefully acknowledged.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Karunya Institute of Technology and Sciences (KITS) through Short Term Research under Grant [number KU/AR/KSTG/34/2017]; Certain basic facilities were shared from the Department of Science and Technology–Science & Engineering Research Board (DST-SERB) under Grant [number SB/YS/LS-252/2013]; and Department of Biotechnology (DBT), Government of India funded projects under Grant [number BT/PR14632/NNT/28/824/2015].