Effect of Sanitizers on Planktonic Edwardsiella tarda Isolated from Asian Stinging Catfish Heteropneustes fossilis (Bloch 1794)

Abstract

Incidence of Edwardsiella tarda in Asian stinging catfish, Heteropneustes fossilis (Bloch), and its sensitivity to antibiotics and sanitizers was studied. Five out of 10 representative lots of H. fossilis were positive for E. tarda. The minimum bactericidal concentration (MBC) of sanitizers (hydrogen peroxide, glutaraldehyde, benzalkonium chloride, sodium hypochlorite, and iodophor) on planktonic E. tarda varied with strains, suspending media (distilled water, physiological saline, pond water, and tryptic soy broth), and combination of factors. The time taken by the sanitizers to achieve 5-log reductions varied greatly between 1 min and 180 min at X and 2X MBCs of sanitizers. Benzalkonium chloride, glutaraldehyde, and sodium hypochlorite were effective in reducing the planktonic E. tarda at concentrations ranging from 3.13 ppm for glutaraldehyde to 25 ppm for sodium hypochlorite. Edwardsiella tarda strains were highly sensitive to ciprofloxacin and exhibited varying degrees of resistance to other antibiotics.

Keywords:

• Heteropneustes fossilis
• Edwardsiella tarda
• sanitizers
• minimum bactericidal concentration
• period of activity
• food safety